LOCUS       40924_10466       10000 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCF430 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Okamoto S, Niki H.
  TITLE     pCF430 vector sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2016) Contact:Sho Okamoto National Institute of 
            Genetics, Microbial Genetics Laboratory, Genetics Strains Reseach 
            Center; 1111 Yata, Mishima, Shizuoka 411-8540, Japan URL 
            :http://www.nig.ac.jp/labs/MicroGen/index.html
REFERENCE   3  (bases 1 to 10000)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-JAN-2016) Contact:Sho Okamoto National Institute of Genetics, 
            Microbial Genetics Laboratory, Genetics Strains Reseach Center; 1111
            Yata, Mishima, Shizuoka 411-8540, Japan URL 
            :http://www.nig.ac.jp/labs/MicroGen/index.html"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             582..1727
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(3040..4119)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4120..4197)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             complement(4259..5455)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     primer_bind     5968..5984
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5985..6041
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(6063..6347)
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             6374..7249
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(7363..7379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7387..7403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7411..7441)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7456..7477)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8932..9653)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     oriT            9798..9907
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 cagacgaacg aagagcgatt gaggaaaagg cggcggcggc cggcatgagc ctgtcggcct
       61 actgctggcc gtcggccagg gctacaaaat cacgggcgtc gtggactatg agcacgtccg
      121 cgagctggcc cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga aactctggct
      181 caccgacgac ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa
      241 gatcgaagag aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc
      301 agagccatga cttttttagc cgctaaaacg gccggggggt gcgcgtgatt gccaagcacg
      361 tccccatgcg ctccatcaag aagagcgact tcgcggagct ggtattcgtg cagggcaaga
      421 ttcggaatac caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg
      481 ataaggtgga ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca
      541 ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc
      601 ggaaggcata caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca
      661 tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg
      721 tccagcaagc tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc
      781 ccgcgccatc ggccgccgtg gagcgttcgc gtcgtctcga acaggaggcg gcaggtttgg
      841 cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg
      901 gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga
      961 agcagcagat caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga
     1021 tgcgagcgat gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa
     1081 tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca
     1141 cctacaccgg cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg
     1201 agtacgcgaa gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc
     1261 aggacctggg ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc
     1321 gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt
     1381 cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc
     1441 tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg
     1501 agaagtaccg caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc
     1561 gggagccgta cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc
     1621 gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc
     1681 tggtggaaca cgcctgggtc aatgatgacc tggtgcattg caaacgctag gggccttgtg
     1741 gggtcagttc cggctggggg ttcagcagcc agcgctttac tggcatttca ggaacaagcg
     1801 ggcactgctc gacgcacttg cttcgctcag tatcgctcgg gcgcacggcg cgctctacga
     1861 actgccgata aacagaggat taaaattgac aattgtgatt aaggctcaga ttcgacggct
     1921 tggagcggcc gacgtgcagg atttccgcga gatccgattg tcggccctga agaaagctcc
     1981 agagatgttc gggtccgttt acgagcacga ggagaaaaag cccatggagg cgttcgctga
     2041 acggttgcga gatgccgtgg cattcggcgc ctacatcgac ggcgagatca ttgggctgtc
     2101 ggtcttcaaa caggaggacg gccccaagga cgctcacaag gcgcatctgt ccggcgtttt
     2161 cgtggagccc gaacagcgag gccgaggggt cgccggtatg ctgctgcggg cgttgccggc
     2221 gggtttattg ctcgtgatga tcgtccgaca gattccaacg ggaatctggt ggatgcgcat
     2281 cttcatcctc ggcgcactta atatttcgct attctggagc ttgttgttta tttcggtcta
     2341 ccgcctgccg ggcgggtcgc ggcgacggta ggcgctgtgc agccgctgat ggtcgtgttc
     2401 atctctgccg ctctgctagg tagcccgata cgattgatgg cggtcctggg ggctatttgc
     2461 ggaactgcgg gcgtggcgct gttggtgttg acaccaaacg cagcgctaga tcctgtcggc
     2521 gtcgcagcgg gcctggcggg ggcggtttcc atggcgttcg gaaccgtgct gacccgcaag
     2581 tggcaacctc ccgtgcctct gctcaccttt accgcctggc aactggcggc cggaggactt
     2641 ctgctcgttc cagtagcttt agtgtttgat ccgccaatcc cgatgcctac aaggaaccaa
     2701 tgttctcggc ctggcgtggc tcggcctgat cggagcgggt ttaacctact tcctttggtt
     2761 ccgggggatc tcgcgactcg aacctacagt tgtttcctta ctgggctttc tcagcccggg
     2821 gaccgccgtc gccgcgctgt tgctaggatg gttgttcttg gatcagacgc tgagtgcgct
     2881 tcaaatcatc ggcgtcctgc tcgtgatcgg gagtgtctgg ctgggccaac gttccaaccg
     2941 cactcctagg gcgcgtatag cttgccggaa gtcgccttga cccgcatggc ataggatata
     3001 ttctagacgc gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     3061 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3121 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     3181 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     3241 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
     3301 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
     3361 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
     3421 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
     3481 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
     3541 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
     3601 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
     3661 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
     3721 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
     3781 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
     3841 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
     3901 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
     3961 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
     4021 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
     4081 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
     4141 ctcttccggg cgctatcatg ccataccgcg aaaggttttg caccattcga tggtgtcaac
     4201 gtaaatgcat gccgcttcgc cttcgcgcgc gaattgcaac ctatcgtttc cacgatcagc
     4261 gatcggctcg ttgccctgcg ccgctccaaa gcccgcgacg cagcgccggc aggcagagca
     4321 agtagagggc agcgcctgca atccatgccc acccgttcca cgttgttata gaagccgcat
     4381 agatcgccgt gaagaggagg ggtccgacga tcgaggtcag gctggtgagc gccgccagtg
     4441 agccttgcag ctgcccctga cgttcctcat ccacctgcct ggacaacatt gcttgcagcg
     4501 ccggcattcc gatgccaccc gaagcaagca ggaccatgat cgggaacgcc atccatcccc
     4561 gtgtcgcgaa ggcaagcagg atgtagcctg tgccgtcggc aatcattccg agcatgagtg
     4621 cccgcctttc gccgagccgg gcggctacag ggccggtgat cattgcctgg gcgagtgaat
     4681 gcagaatgcc aaatgcggca agcgaaatgc cgatcgtggt cgcgtcccag tgaaagcgat
     4741 cctcgccgaa aatgacccaa agcgcggccg gcacctgtcc gacaagttgc atgatgaaga
     4801 agaccgccat cagggcggcg acgacggtca tgccccgggc ccaccggaac gaagcgagcg
     4861 ggttgagagc ctcccggcgt aacggccggc gttcgccttt gtgcgactcc ggcaaaagga
     4921 aacagcccgt caggaaattg aggccgttca aggctgccgc ggcgaagaac ggagcgtggg
     4981 gggagaaacc gcccatcagc ccaccgagca caggtcccgc gaccatcccg aacccgaaac
     5041 aggcgctcat gaagccgaag tgccgcgcgc gctcatcgcc atcagtgata tcggcaatat
     5101 aagcgccggc taccgcccca gtcgccccgg tgatgccggc cacgatccgc ccgatataga
     5161 gaacccaaag gaaaggcgct gtcgccatga tggcgtagtc gacagtggcg ccggccagcg
     5221 agacgagcaa gattggccgc cgcccgaaac gatccgacag cgcgcccagc acaggtgcgc
     5281 aggcaaattg caccaacgca tacagcgcca gcagaatgcc atagtgggcg gtgacgtcgt
     5341 tcgagtgaac cagatcgcgc aggaggcccg gcagcaccgg cataatcagg ccgatgccga
     5401 cagcgtcgag cgcgacagtg ctcagaatta cgatcagggg tatgttgggt ttcacgtctg
     5461 gcctccggac cagcctccgc tggtccgatt gaacgcgcgg attctttatc actgataagt
     5521 tggtggacat attatgttta tcagtgataa agtgtcaagc atgacaaagt tgcagccgaa
     5581 tacagtgatc cgtgccgccc tggacctgtt gaacgaggtc ggcgtagacg gtctgacgac
     5641 acgcaaactg gcggaacggt tgggggttca gcagccggcg ctttactggc acttcaggaa
     5701 caagcgggcg ctgctcgacg cactggccga agccatgctg gcggagaatc atacgcattc
     5761 ggtgccgaga gccgacgacg actggcgctc atttctgatc gggaatgccc gcagcttcag
     5821 gcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     5881 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aacgcgatta agttgggtaa
     5941 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta
     6001 cccggggatc ctctagagtc gacctgcagg catgcaagct tcgctagccc aaaaaaacgg
     6061 gtatggagaa acagtagaga gttgcgataa aaagcgtcag gtaggatccg ctaatcttat
     6121 ggataaaaat gctatggcat agcaaagtgt gacgccgtgc aaataatcaa tgtggacttt
     6181 tctgccgtga ttatagacac ttttgttacg cgtttttgtc atggctttgg tcccgctttg
     6241 ttacagaatg cttttaataa gcggggttac cggtttggtt agcgagaaga gccagtaaaa
     6301 gacgcagtga cggcaatgtc tgatgcaata tggacaattg gtttcttctc tgaatggcgg
     6361 gagtatgaaa agtatggctg aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa
     6421 tgcccatctg gtggcgggtt taacgccgat tgaggccaac ggttatctcg atttttttat
     6481 cgaccgaccg ctgggaatga aaggttatat tctcaatctc accattcgcg gtcagggggt
     6541 ggtgaaaaat cagggacgag aatttgtttg ccgaccgggt gatattttgc tgttcccgcc
     6601 aggagagatt catcactacg gtcgtcatcc ggaggctcgc gaatggtatc accagtgggt
     6661 ttactttcgt ccgcgcgcct actggcatga atggcttaac tggccgtcaa tatttgccaa
     6721 tacggggttc tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc tgtttgggca
     6781 aatcattaac gccgggcaag gggaagggcg ctattcggag ctgctggcga taaatctgct
     6841 tgagcaattg ttactgcggc gcatggaagc gattaacgag tcgctccatc caccgatgga
     6901 taatcgggta cgcgaggctt gtcagtacat cagcgatcac ctggcagaca gcaattttga
     6961 tatcgccagc gtcgcacagc atgtttgctt gtcgccgtcg cgtctgtcac atcttttccg
     7021 ccagcagtta gggattagcg tcttaagctg gcgcgaggac caacgtatca gccaggcgaa
     7081 gctgcttttg agcaccaccc ggatgcctat cgccaccgtc ggtcgcaatg ttggttttga
     7141 cgatcaactc tatttctcgc gggtatttaa aaaatgcacc ggggccagcc cgagcgagtt
     7201 ccgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc aagttgtcat aattggtaac
     7261 gaatcagaca attgacggct tgacggagta gcatagggtt tgcagaatcc ctgcttcgtc
     7321 catttgacag gcacattatg catcgatgaa ttcgtaatca tggtcatagc tgtttcctgt
     7381 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7441 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7501 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7561 aggcggtttg cgtattgggc gctgctcgcc taccgcgatg gcgcgcgcat ccatgccggc
     7621 acgcgaccgg gcgcaccgca gatggaaacg gccgacgcgc agcttcgctt cctctgcgag
     7681 gcgggttttt cggccgggga cgccgtcaat gcgctgatga caatcagcta cttcactgtt
     7741 ggggccgtgc ttgaggagca tgccggcgac agcgatgccg gcgagcgcgg cggcaccgtt
     7801 gaacaggctc cgctctcgcc gctgttgcgg gccgcgatag acgccttcga cgaagccggt
     7861 ccggacgcag cgttcgagca gggactcgcg gtgattgtcg atggattggc gaaaaggagg
     7921 ctcgttgtca ggaacgttga aggaccgaga aagggtgacg attgatcagg accgctgccg
     7981 gagcgcaacc cactcactac agcagagcca tgtagacaac atcccctccc cctttccacc
     8041 gcgtcagacg cccgtagcag cccgctacgg gctttttcat gccctgccct agcgtccaag
     8101 cctcacggcc gcgctcggcc tctctggcgg ccttctggcg ctcctgctgc ggcgtccgct
     8161 cgtgggccgt ggcgcgggtc cgcgcgccgg cctcgtgcgc ctggcgctcg cgggcgaggt
     8221 ccagggcggc cgtcttcacg ttctgccttg cgcagatgag atagatccag tctgcaagtg
     8281 tcagaaacga gtctgcattt gatgccctga acgtcttcat acatctgata agcccctccc
     8341 ggaagtctgc tttctctccg tcagaaagca taccctgacc ggtttcaatc acggaccgtt
     8401 ctgtcaggta atgctctgtc cagtcataca gagccgggtt tgtcacccgt ataagctgaa
     8461 gcaggcacaa atcagggaaa taaacaaaat cccgcatccc cggataaaga aaaatcaggg
     8521 aattaatggc ctgatggatt tcccgtggcg tcgaaagtgc ggcaccatag gtatcagtca
     8581 ccgcgatgag atcccttacc attccagagt ctggcggttg attattaatt tgctgatata
     8641 gagcctcagc ccgctggcga aattcattac gtaaatcaaa ggcttcaggt cggggtaatt
     8701 taaaactaag ctgaatgatt ttctggagat aacggctgcc atcttcgata ttcagcgcat
     8761 gttcaacggc atgagtgata atctgcctgt cataacagag aatatgggta aagcggggca
     8821 gatcggctac tgcacgcaca agcctgaaca cttccgccac ctgggatggc tccagtcggt
     8881 ccagatcatc catgacaaca atgaacttca gatccagact caccagttgt cctgccattt
     8941 ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg aggcccgcgt
     9001 tagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg cggtcacgcg
     9061 cacagggcgc agcacagggc gcagccctgg ttaaaaacaa ggtttataaa tattggttta
     9121 aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg
     9181 ctggattttc tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc tcatctgtca
     9241 gcactctgcc cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc
     9301 aagtgtcaat accgcagggc acttatcccc aggcttgtcc acatcatctg tgggaaactc
     9361 gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc agctccacgt cgccggccga
     9421 aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca
     9481 acgtccgccc ctcatctgtc agtgagggcc aagttttccg cgaggtatcc acaacgccgg
     9541 cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag
     9601 acggccgcca gcccagcggc gagggcaacc agcccggtga gcgtcggaaa gggtcgacgg
     9661 atcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
     9721 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
     9781 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
     9841 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
     9901 tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca
     9961 accaggaagg gcagcccacc tatcaaggtg tactgccttc
//
